Ability to: select, fit and use personal protective clothing and/or equipment, including gowning up, following required work area entry and exit procedures and moving around the work area to minimise risk of contamination prepare equipment for operation, such as inspecting equipment condition to identify any signs of wear, disinfecting and sterilising equipment and surfaces, assembling columns, confirming that connections and valves are correctly positioned, ensuring column lines have been purged and all safety guards are in place and operational, selecting instrumentation settings, cancelling isolation or lock outs as required, and conducting pre-start checks to check equipment readiness carry out sample testing to confirm integrity of columns (this is typically done by conducting a Height Equivalent to Theoretical Plate (HETP) test) demonstrate procedure for loading/packing product into columns start, operate, and monitor the process to achieve required outcomes, including monitoring: chromatography cycles correct collection of fractions as required appropriate product segregation pump operation take corrective action in response to out-of-specification results maintain security, integrity and traceability of samples, sub-samples and documentation respond to and/or report equipment failure within level of responsibility locate emergency stop functions on equipment follow isolation and lock out/tag out procedures as required to take process and related equipment off line in preparation for cleaning and/or maintenance within level of responsibility demonstrate product/process changeovers, including demonstrating column storage procedures follow end of batch procedures including line clearance and cleaning, yield calculation, materials reconciliation and product labelling complete workplace records as required maintain work area to meet housekeeping standards use oral communication skills/language competence to fulfil the job role as specified by the organisation, including questioning, active listening, asking for clarification and seeking advice from supervisor work cooperatively within a culturally diverse workforce |