Elements and Performance Criteria
- Prepare for aseptic sampling or transfer
- Ensure that any sampling procedure conforms with the requirements of the sampling plan
- Use specified personal protective clothing and equipment
- Prepare the work area for safe and effective sample transfer
- Select equipment and materials specified by the procedure
- Organise equipment to minimise contamination during manipulations
- Label containers for clear identification
- Record details in relevant log or database
- Transfer materials aseptically
- Protect the integrity of the sample source by sterilising the sampling site and the mouth of transport or culture vessel
- Sterilise inoculating loops and/or pipette where used to prevent contamination
- Perform transfer while minimising opportunities for contamination and cross-infection
- After transfer, and before sealing the transport or culture vessel, flame the vessel mouth to maintain sterility
- Re-sterilise inoculating loops, minimising the generation of aerosols
- Perform quality control checks, if required
- Label transport or culture vessels for clear identification
- Maintain work area and equipment to prevent cross-infection and contamination