Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1

Interpret and schedule production requirements

1.1

Review client request and confirm quantity and nature of cells, tissue or products

1.2

Select appropriate media, materials, equipment and methods

1.3

Plan parallel work sequences to optimise production

1.4

Maintain a chain of custody, traceable to the worker, for all cells and tissues

2

Work safely according to the legal and regulatory framework

2.1

Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and equipment

2.2

Select, fit and use personal protective equipment (PPE)

2.3

Address hazards and incidents as they arise

2.4

Safely dispose of biohazardous materials and other laboratory waste

3

Assemble and maintain tissue culture equipment

3.1

Assemble, sterilise or decontaminate equipment according to workplace procedures

3.2

Perform pre-use and safety checks in accordance with relevant workplace and operating procedures

3.3

Identify faulty or unsafe components and equipment and report to appropriate personnel

3.4

Decontaminate area and equipment after use

4

Prepare and test cell and tissue culture media

4.1

Confirm media specifications and processes/methods

4.2

Prepare culture media to suit client request

4.3

Sterilise culture media and check for sterility

4.4

Perform quality control checks to ensure that culture media is fit for purpose

4.5

Store culture media in accordance with specifications

5

Obtain, monitor and maintain tissue and cell lines

5.1

Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture

5.2

Select specified culture media and add any necessary growth agents or nutrients

5.3

Incubate cells/tissue in specified conditions

5.4

Inoculate the media with the specified amount of sample

5.5

Monitor growth of tissue and cell lines and products

5.6

Detect contamination/abnormalities and troubleshoot materials, equipment and techniques

5.7

Passage samples by subculturing to preserve or grow the line

5.8

Harvest cells or cell products to optimise yields

6

Preserve cells and tissues

6.1

Select the appropriate preservation method

6.2

Preserve the cell lines or tissue in accordance with the method

6.3

Check preserved cell lines regularly to ensure viability is maintained

7

Maintain records

7.1

Maintain records of batches of media

7.2

Maintain records of active and stored tissue and cell lines

7.3

Complete records so that they are retrievable, legible and accurate

7.4

Ensure records conform to the information management, records, quality system and legal requirements