Apply advanced embedding and microtomy skills

Formats and tools

Unit Description
Reconstruct the unit from the xml and display it as an HTML page.
Assessment Tool
an assessor resource that builds a framework for writing an assessment tool
Assessment Template
generate a spreadsheet for marking this unit in a classroom environment. Put student names in the top row and check them off as they demonstrate competenece for each of the unit's elements and performance criteria.
Assessment Matrix
a slightly different format than the assessment template. A spreadsheet with unit names, elements and performance criteria in separate columns. Put assessment names in column headings to track which performance criteria each one covers. Good for ensuring that you've covered every one of the performance criteria with your assessment instrument (all assessement tools together).
Wiki Markup
mark up the unit in a wiki markup codes, ready to copy and paste into a wiki page. The output will work in most wikis but is designed to work particularly well as a Wikiversity learning project.
Evidence Guide
create an evidence guide for workplace assessment and RPL applicants
Competency Mapping Template
Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners. A template for developing assessments for a unit, which will help you to create valid, fair and reliable assessments for the unit, ready to give to trainers and students
Observation Checklist
create an observation checklist for workplace assessment and RPL applicants. This is similar to the evidence guide above, but a little shorter and friendlier on your printer. You will also need to create a seperate Assessor Marking Guide for guidelines on gathering evidence and a list of key points for each activity observed using the unit's range statement, required skills and evidence required (see the unit's html page for details)

Self Assessment Survey
A form for students to assess thier current skill levels against each of the unit's performance criteria. Cut and paste into a web document or print and distribute in hard copy.
Moodle Outcomes
Create a csv file of the unit's performance criteria to import into a moodle course as outcomes, ready to associate with each of your assignments. Here's a quick 'how to' for importing these into moodle 2.x
Registered Training Organisations
Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver MSL975028, 'Apply advanced embedding and microtomy skills'.
Google Links
links to google searches, with filtering in place to maximise the usefulness of the returned results
Books
Reference books for 'Apply advanced embedding and microtomy skills' on fishpond.com.au. This online store has a huge range of books, pretty reasonable prices, free delivery in Australia *and* they give a small commission to ntisthis.com for every purchase, so go nuts :)


Elements and Performance Criteria

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1

Prepare specialised tissues

1.1

Prepare agar plugs for cytological or minute specimens

1.2

Prepare blood clot for processing

1.3

Prepare friable /tiny tissue in sponges and/or wrapped to ensure tissue integrity and prevent loss during processing

1.4

Decalcify bone

2

Embed Tissue

2.1

Perform equipment pre-use check on embedding centre to ensure samples integrity and uninterrupted embedding of processor load

2.2

Identify and prioritise specimens prior to embedding

2.3

Check tissue for complete processing prior to embedding

2.4

Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy

2.5

Select appropriately sized mould

2.6

Apply the use of an embedding run-sheet to check the number of pieces of tissue in each cassette and noting any discrepancies

2.7

Apply procedures to prevent cross-contamination between tissues

2.8

Embed tissue with embedding orientation markings

2.9

Perform excess wax removal from blocks using manual scrapping and/or heat removal

2.10

Inspect blocks, reject and re-embed items that do not meet quality control standards

2.11

Apply quality control procedures to troubleshoot discrepancies between run-sheet and tissue embedded

2.12

File and store tissue blocks to facilitate efficient retrieval as required

3

Cut tissue sections

3.1

Check that flotation bath is ready and satisfactory for use

3.2

Prepare and adjust all external parts of a microtome and associated equipment to accommodate requirements of tissue batch

3.3

Secure block in microtome following specified safety directions

3.4

Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts

3.5

Apply microtomy techniques to trim blocks to full face without significant loss of tissue

3.6

Cut thin tissue sections according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing

3.7

Float ribbons onto water bath to flatten sections

3.8

Pick up sections onto microscope slides ensuring patient identification on slides matches what is on block

3.9

Apply procedures to prevent cross-contamination between patient tissues

3.10

Compare the blocks and sections ensuring the tissue on slides matches that in the blocks

3.11

Inspect sections and reject any that do not meet quality control standards

3.12

Identify, troubleshoot and resolve common section quality control issues

3.13

Apply quality control procedures to troubleshoot discrepancies between run-sheet and sections on slides